Reporter
Part:BBa_K2675006:Design
Designed by: Esteban Lebrun Group: iGEM18_Evry_Paris-Saclay (2018-10-05)
sfGFP-LVAtag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 25
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Coding sequence of superfolder GFP (BBa_K2675005) + LVA degradation tag sequence (AANDENYALVA).
Source
DNA synthesis
References
[1] Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol (2006) 24, 79-88.
[2] Purcell O, Grierson CS, Bernardo Md, Savery NJ. Temperature dependence of ssrA-tag mediated protein degradation. J Biol Eng (2012) 6, 10.