Reporter

Part:BBa_K2675006:Design

Designed by: Esteban Lebrun   Group: iGEM18_Evry_Paris-Saclay   (2018-10-05)


sfGFP-LVAtag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequence of superfolder GFP (BBa_K2675005) + LVA degradation tag sequence (AANDENYALVA).

Source

DNA synthesis

References

[1] Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol (2006) 24, 79-88.

[2] Purcell O, Grierson CS, Bernardo Md, Savery NJ. Temperature dependence of ssrA-tag mediated protein degradation. J Biol Eng (2012) 6, 10.